MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02879nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625160158.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16786 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Optimization for Guide RNA and CRISPR/Cas9 System Nanoparticle Mediated Delivery into Plant Cell for Genome Editing |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
World Academy of Science, Engineering and Technology International Journal of Agricultural and Biosystems Engineering, 12(3), p.51, 2018 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Due to its simplicity, CRISPR/Cas9 has become widely used and capable of inducing mutations in the genes of organisms of various kingdoms. The aim of this work was to develop applications for the efficient modification of DNA coding sequences of phytoene desaturase (PDS), coilin and vacuolar invertase (Solanum tuberosum)genes, and to develop a new nanoparticles carrier efficient technology to deliver the CRISPR/Cas9 system for editing the plant genome. For each of the genes - coilin, PDS and vacuolar invertase, five single RNA guide (sgRNAs)were synthesized. To determine the most suitable nanoplatform, two types of NP platforms were used: magnetic NPs (MNPS)and gold NPs (AuNPs). To test the penetration efficiency, they were functionalized with fluorescent agents - BSA * FITS and GFP, as well as labeled Cy3 small-sized RNA. To measure the efficiency, a fluorescence and confocal microscopy were used. It was shown that the best of these options were AuNP - both in the case of proteins and in the case of RNA. The next step was to check the possibility of delivering components of the CRISPR/Cas9 system to plant cells for editing target genes. AuNPs were functionalized with a ribonucleoprotein complex consisting of Cas9 and corresponding to target genes sgRNAs, and they were biolistically bombarded to axillary buds and apical meristems of potato plants. After the treatment by the best NP carrier, potato meristems were grown to adult plants. DNA isolated from this plants was sent to a preliminary fragment of the analysis to screen out the non-transformed samples, and then to the NGS. The present work was carried out with the financial support from the Russian Science Foundation (grant No. 16-16-04019) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
BIOBOMBARDMENT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
COILIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CRISPR/CAS9 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
NANOPARTICLES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
NPS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SGRNA, VACUOLAR INVERTASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Khromov, Andrey V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Makhotenko, Antonida V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Snigir, Ekaterina A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Makarova, Svetlana S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kalinina, Natalia O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Makarov, Valentin V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Taliansky, Mikhail E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1b5nyfMNnTyvsEDlJsywv6ZJHE9EdAKZT/view?usp=drivesdk">https://drive.google.com/file/d/1b5nyfMNnTyvsEDlJsywv6ZJHE9EdAKZT/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
