Expression of defence-related genes in stems and leaves of resistant and susceptible field pea (Pisum sativum)during infection by Phoma koolunga (Record no. 50958)

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fixed length control field 02570nam a2200241Ia 4500
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control field MX-MdCICY
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control field 20250625160158.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16791
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245 10 - TITLE STATEMENT
Title Expression of defence-related genes in stems and leaves of resistant and susceptible field pea (Pisum sativum)during infection by Phoma koolunga
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Pathology, 67(1), p.156-166, 2018
520 3# - SUMMARY, ETC.
Summary, etc. The differential expression of 13 defence-related genes during Phoma koolunga infection of stems and leaves of susceptible versus resistant field pea (Pisum sativum)was determined using qRT-PCR. Expression, in terms of relative mRNA level ratios, of genes encoding ferredoxin NADP oxidoreductase, 6a-hydroxymaackiain methyltransferase (hmm6), chalcone synthase (PSCHS3)and ascorbate peroxidase in leaves and stems differed during 6-72 hours post-inoculation (hpi)and reflected known host resistance levels in leaves versus stems. In comparison to the susceptible genotype, at 24, 48 and 72 hpi, two genes, hmm6 (122.43-, 206.99- and 32.25-fold, respectively)and PSCHS3 (175.00-, 250.13- and 216.24-fold, respectively), were strongly up-regulated in leaves of the resistant genotype, highlighting that resistance against P. koolunga in field pea is governed by the early synthesis of pisatin. At 24 hpi, leaves infected by P. koolunga showed clear differences in expression of target genes. For example, the gene encoding a precursor of the defensin 'disease resistance response protein 39' was substantially down-regulated in leaves of both the susceptible and the resistant genotypes inoculated with P. koolunga. This contrasts with other studies on another pea black spot pathogen, Didymella pinodes, where this same gene is strongly up-regulated in leaves of resistant and susceptible genotypes. The current study provides the first understanding of defence-related genes involved in the resistance against P. koolunga, opening novel avenues to engineer new field pea cultivars with improved leaf and stem black spot disease resistance as the basis for developing more effective and sustainable management strategies.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASCOCHYTA BLIGHT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HOST RESISTANCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PEA BLACK SPOT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RESISTANCE MECHANISMS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tran, H.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name You, M.P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Barbetti, M.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Hdrjka103kULxYmS5xdkL8Xw-90mBvJ5/view?usp=drivesdk">https://drive.google.com/file/d/1Hdrjka103kULxYmS5xdkL8Xw-90mBvJ5/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16791 25.06.2025 25.06.2025 Documentos solicitados