MARC details
| 000 -LEADER |
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| fixed length control field |
02648nam a2200469Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160158.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16798 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Cell, 171(7), p.1495-1507.e15, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Current genome-editing systems generally rely on inducing DNA double-strand breaks (DSBs). This may limit their utility in clinical therapies, as unwanted mutations caused by DSBs can have deleterious effects. CRISPR/Cas9 system has recently been repurposed to enable target gene activation, allowing regulation of endogenous gene expression without creating DSBs. However, in vivo implementation of this gain-of-function system has proven difficult. Here, we report a robust system for in vivo activation of endogenous target genes through trans-epigenetic remodeling. The system relies on recruitment of Cas9 and transcriptional activation complexes to target loci by modified single guide RNAs. As proof-of-concept, we used this technology to treat mouse models of diabetes, muscular dystrophy, and acute kidney disease. Results demonstrate that CRISPR/Cas9-mediated target gene activation can be achieved in vivo, leading to measurable phenotypes and amelioration of disease symptoms. This establishes new avenues for developing targeted epigenetic therapies against human diseases. Video Abstract In vivo delivery of a Cas9-based epigenetic gene activation system ameliorates disease phenotypes in mouse models of type I diabetes, acute kidney injury, and muscular dystrophy |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHROMATIN REMODELING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRISPR/CAS9 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DISEASE MODEL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EPIGENETIC MODIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EPIGENETIC THERAPY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENE EDITING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MUSCULAR DYSTROPHY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
REGENERATIVE MEDICINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
STEM CELLS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANSDIFFERENTIATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Liao, H.-K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hatanaka, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Araoka, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Reddy, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wu, M.-Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Sui, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yamauchi, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yamauchi, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
O'Keefe, D.D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Núñez-Delicado, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Guillen, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Campistol, J.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wu, C.-J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lu, L.-F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Esteban, C.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Izpisua Belmonte, J.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1XHJr6DfWyZ7Jly9U3ZTTCWP80qgHSqae/view?usp=drivesdk">https://drive.google.com/file/d/1XHJr6DfWyZ7Jly9U3ZTTCWP80qgHSqae/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
