MARC details
| 000 -LEADER |
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02683nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160158.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16809 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
BioChemical Journal, 445(3), p.337-347, 2012 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Plants contain both cytosolic and chloroplastic GAPDHs (glyceraldehyde-3- phosphate dehydrogenases). In Arabidopsis thaliana, cytosolic GAPDH is involved in the glycolytic pathway and is represented by two differentially expressed isoforms (GapC1 and GapC2)that are 98 percent identical in amino acid sequence. In the present study we show that GapC1 is a phosphorylating NAD-specific GAPDH with enzymatic activity strictly dependent on Cys149. Catalytic Cys 149 is the only solvent-exposed cysteine of the protein and its thiol is relatively acidic (pKa = 5.7). This property makes GapC1 sensitive to oxidation by H2O2, which appears to inhibit enzyme activity by converting the thiolate of Cys149 (-S-)into irreversible oxidized forms (-SO2 - and -SO 3 -)via a labile sulfenate intermediate (-SO-). GSH (reduced glutathione)prevents this irreversible process by reacting with Cys149 sulfenates to give rise to a mixed disulfide (Cys 149-SSG), as demonstrated by both MS and biotinylated GSH. Glutathionylated GapC1 can be fully reactivated either by cytosolic glutaredoxin, via a GSH-dependent monothiol mechanism, or, less efficiently, by cytosolic thioredoxins physiologically reduced by NADPH:thioredoxin reductase. The potential relevance of these findings is discussed in the light of the multiple functions of GAPDH in eukaryotic cells (e.g. glycolysis, control of gene expression and apoptosis)that appear to be influenced by the redox state of the catalytic Cys149. © The Authors Journal compilation |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ARABIDOPSIS THALIANA CYTOSOLIC ISOFORM 1 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (ATGAPC1) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DEGLUTATHIONYLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GLUTATHIONYLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GLYCOLYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
OXIDATIVE STRESS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
REDOX SIGNALLING |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bedhomme, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Adamo, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Marchand, C.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Couturier, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rouhier, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lemaire, S.D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zaffagnini, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zaffagnini, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1xuZHXcSFA53GokKDyAx73eq6y_W474zJ/view?usp=drivesdk">https://drive.google.com/file/d/1xuZHXcSFA53GokKDyAx73eq6y_W474zJ/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
