Secuenciación del genoma completo del potato yellow vein virus (PYVV)en tomate (solanum lycopersicum)en Colombia = Genome sequencing of potato yellow vein virus (PYVV)strain infecting solanum lycopersicum in Colombia (Record no. 51072)

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fixed length control field 02094nam a2200253Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160200.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16907
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245 10 - TITLE STATEMENT
Title Secuenciación del genoma completo del potato yellow vein virus (PYVV)en tomate (solanum lycopersicum)en Colombia = Genome sequencing of potato yellow vein virus (PYVV)strain infecting solanum lycopersicum in Colombia
490 0# - SERIES STATEMENT
Volume/sequential designation Acta Biologica Colombiana, 22(1), p.5-17, 2017
520 3# - SUMMARY, ETC.
Summary, etc. Potato yellow vein virus (PYVV)is one of the most important pathogens of potato in the Andean region. In spite of having been detected in tomato crops in Colombia, knowledge on the biological characteristics of PYVV is limited on this host. In this study, next-generation sequencing (NGS)of a PYVV strain infecting tomato in Marinilla District (Antioquia)was performed; additionally, three primer set useful in RT-PCR and RT-qPCR detection were also tested. The consensus genome consisted of three RNA segments of 8043 nt (RNA1), 5346 nt (RNA2)and 3896 nt (RNA3)encoding ten ORF with slight lower sequence identity in relation to PYVV isolates from potato. Sequence analysis suggests the presence of regions potentially undergoing positive selection in the ORFs coding for MET/HEL and CPm possibly as a result of host adaptation. Experimental validation of primers resulted in amplicon with the expected size while melting temperature analysis and sequencing suggest the presence of at least two PYVV infecting S. lycopersicum in east Antioquia in agreement with the NGS data. © 2016, Universidad Nacional de Colombia
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRINIVIRUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RT-PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RT-QPCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOLANACEAE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Muñoz Baena, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gutiérrez Sánchez, P.A
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marín Montoya, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ZZduCpIJ8mIAoQR7y87CzChBqJjTsH6a/view?usp=drivesdk">https://drive.google.com/file/d/1ZZduCpIJ8mIAoQR7y87CzChBqJjTsH6a/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16907 25.06.2025 25.06.2025 Documentos solicitados