Transformación de embriones somáticos de mango por biobalística = Transformation of mango somatic embryos by biobalistics (Record no. 51075)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160200.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16910
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245 10 - TITLE STATEMENT
Title Transformación de embriones somáticos de mango por biobalística = Transformation of mango somatic embryos by biobalistics
490 0# - SERIES STATEMENT
Volume/sequential designation Interciencia, 29(5), p.261-266+231, 2004
520 3# - SUMMARY, ETC.
Summary, etc. In order to genetically transform mango by biobalistic, somatic embryos of the varieties Haden, Madame Francis and Kent were bombarded. The maximum dose of PPT that somatic embryos survive was determined by exposing them in Gamborg, Miller y Ojima media containing 0, 0.5, 1 and 2mg/l PPT during 3 months and subculturing every month to evaluate fresh and dry weight. The embryos were bombarded with the plasmid GAMBIA 3201 with genes GUS y BAR, using two tungsten particle sizes (0.7 and 1.3?m), pressure of 80psi, bombardment distances of 10 and 16.5cm, and 5?g of DNA. Embryos were selected using 0.5mg/l PPT as selective agent. Particle size of 0.7?m and the bombardment distance of 16.5cm were selected. After 3 months of selection in the indicated PPT concentrations, selected clones of the Kent variety survived all concentrations, while non-transformed embryos survived only at 0.5mg/l. Haden and Madame Francis varieties were sensitive to the bombardment and selection conditions, and did not survive the process. Embryo survival was 4 percent, and 3 clones per 0.5g of tissues bombarded were regenerated. Enzymatic activity of transient expression of the GUS gene was observed and the incorporation of the GUS and BAR genes was demonstrated by means of PCR.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CLONES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENETIC TRANSFORMS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chavarri, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vegas, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zambrano, A.Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Demey, J.R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1KLTV_Y2_8N5j3zMJvSboOMLUCdKkCUfl/view?usp=drivesdk">https://drive.google.com/file/d/1KLTV_Y2_8N5j3zMJvSboOMLUCdKkCUfl/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16910 25.06.2025 25.06.2025 Documentos solicitados