Effective selection and regeneration of transgenic sugarcane plants using positive selection system (Record no. 51078)

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fixed length control field 01677nam a2200229Ia 4500
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control field MX-MdCICY
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control field 20250625160200.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16913
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Title Effective selection and regeneration of transgenic sugarcane plants using positive selection system
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Volume/sequential designation In Vitro Cellular & Developmental Biology-Plant, 51(1), p.52-61, 2015
520 3# - SUMMARY, ETC.
Summary, etc. We compared the transformation frequency of a positive selection system, which employs an Escherichia coli-derived pmi (phosphomannose isomerase)gene as the selectable marker and mannose as the selective agent, to commonly used negative selection systems of bar (phosphinothricin acetyltransferase)/phosphinothricin (PPT), npt II (neomycin phosphotransferase)/G418 (geneticin), and hpt (hygromycin phosphotransferase)/Hygromycin. Transgenic sugarcane plants were obtained following biolistic transformation of callus with four different selectable marker genes. Under different selective agents, transformed callus was selectively proliferated on MS medium containing 13.57 µM of 2,4-D, regenerated on MS medium with 4.44 µM of BA, 9.29 µM of KT, and 0.89 µM of NAA, and rooted on half of MS medium with 13.38 µM of NAA and 0.44 µM of 6-BA. The results demonstrated that the transformation frequency of 4.2
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, M.
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Personal name Zhuo, X.
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Personal name Wang, J.
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Personal name Wu, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yao, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chen, R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/19Njg5yzcG0o0J4BJb1dF46EtPcFceAKG/view?usp=drivesdk">https://drive.google.com/file/d/19Njg5yzcG0o0J4BJb1dF46EtPcFceAKG/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16913 25.06.2025 25.06.2025 Documentos solicitados