MARC details
| 000 -LEADER |
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| fixed length control field |
02739nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160200.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16914 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
High-efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markers |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Molecular Breeding, 2(3), p.239-249, 1996 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Transient expression of the maize anthocyanin regulatory elements, R and C1, was used to optimise parameters for microprojectile-mediated delivery of DNA into sugarcane embryogenic callus. Osmotic treatment of target tissues and particle acceleration in a high-pressure helium pulse increased the frequency of transient expression to 5-8 × 103 cells per bombardment, with minimal tissue damage. An average of 0.34 percent of transiently expressing cells developed into stably transformed, anthocyanin-pigmented proembryoids which subsequently regenerated into plantlets. However, constitutive expression of R and C1 proved deleterious, and no anthocyanin-pigmented plant survived beyond 3 cm in height. We also compared selective subculture of callus portions showing luciferase activity with antibiotic selection on medium containing G418 or phosphinothricin, upon bombardment of callus with constructs driving strong expression of luc, aphA or bar genes. Selective subculture based on luciferase activity enabled recovery of 1.4 ± 0.5 independent transgenic plants per bombardment, compared to 19.8 ± 3.7 independent transgenic plants per bombardment from an optimised G418 selection regimen, and no transformed plants from phosphinothricin selection. When luc and aphA on separate plasmids were coprecipitated onto microprojectiles before bombardment, 67-79 percent of callus lines selected for G418 resistance also showed luciferase activity detectable under a low-light camera. Southern analysis confirmed a very high cotransformation frequency, with variable copy numbers of introduced genes. The high efficiencies of gene transfer, selection and cotransformation in the optimised system, coupled with the simple initiation and regeneration of embryogenic callus, provide an effective tool for practical genetic transformation of sugarcane. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTHOCYANIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIBIOTIC G418 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LUCIFERASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PHOSPHINOTHRICIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SACCHARUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANSFORMATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bower, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Elliott, A.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Potier, B.A.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Birch, R.G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1h3VZJxOgm4zl9u-scKaJn7Lb2uSVuFjy/view?usp=drivesdk">https://drive.google.com/file/d/1h3VZJxOgm4zl9u-scKaJn7Lb2uSVuFjy/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
