Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane (Record no. 51081)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01874nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160200.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16916 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Cell Reports, 19(12), p.1188-1194, 2000 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Embryogenic calli of the Brazilian sugarcane (Saccharum officinarum L.)genotype SP80-180 were transformed with two plasmids containing genes coding for neomycin phosphotransferase (neo)and phosphinotrycin acetyltransferase (bar), by particle bombardment using an apparatus developed at Copersucar Technology Center. Transformed plants were initially selected on culture medium containing Geneticin, and resistance was confirmed by localized application of a kanamycin solution to leaves of hardened plants at the nursery. A commercial formulation of ammonium gluphosinate was sprayed twice on these antibiotic-resistant plants. The resistant plants were considered co-transformed, and Southern analysis confirmed stable integration of both bar and neo genes. In addition, phosphinotrycin acetyltransferase expression was supported by RT-PCR analysis and neomycin phosphotransferase presence was demonstrated by western blotting. Similar analyses were also performed with micropropagated transformants after three cycles of subculture. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NEOMYCIN PHOSPHOTRANSFERASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PARTICLE BOMBARDMENT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHOSPHINOTRYCIN ACETYLTRANSFERASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSGENIC SUGARCANE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Falco, M.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tulmann Neto, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ulian, E.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1t3WXOAOw9o-igyWj7I8ditAQS6K4Mbw0/view?usp=drivesdk">https://drive.google.com/file/d/1t3WXOAOw9o-igyWj7I8ditAQS6K4Mbw0/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16916 | 25.06.2025 | 25.06.2025 | Documentos solicitados |