Herbicide resistant transgenic sugarcane plants containing the bar gene (Record no. 51082)

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fixed length control field 02122nam a2200217Ia 4500
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control field MX-MdCICY
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control field 20250625160200.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16917
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245 10 - TITLE STATEMENT
Title Herbicide resistant transgenic sugarcane plants containing the bar gene
490 0# - SERIES STATEMENT
Volume/sequential designation Crop Science, 36(5), p.1367-1374, 1996
520 3# - SUMMARY, ETC.
Summary, etc. We have obtained herbicide resistant transgenic sugarcane plants (Saccharum spp. hybrids)of the commercial cultivar NCo 310. A particle inflow gun was used to bombard embryogenic calli obtained from immature inflorescences with microprojectiles coated with a plasmid containing the coding region of the bar gene fused to the maize ubiquitin-1 (Ubi-1)promoter, first exon, and first intron. Shoots were regenerated from calli selected for resistance to bialaphos (1 mg/L), and rooted on herbicide- containing medium (3 mg/L). Plants regenerated on selective media were screened for the presence of the bar gene by polymerase chain reaction. Northern blot analysis showed that transgenics were producing chimeric transcripts of the proper size. Most of the transformants examined displayed resistance to the commercial herbicide. Ignite applied either to leaf segments or to the entire plant at dosages lethal to nontransformed controls. Southern blot analysis confirmed stable integration of three to 10 copies of the transgene into the sugarcane genome. A well-characterized transformant was submitted to three rounds of vegetative propagation, as well as through meristem culture. All resulting propagated clones displayed herbicide resistance comparable to the original transformant. The above results demonstrate that the bar gene can be an effective selectable marker in sugarcane transformation studies.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SACCHARUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SACCHARUM HYBRID CULTIVAR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ZEA MAYS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gallo-Meagher, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Irvine, J.E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1FSKSZpPxg2wVF0yYw9wnl_KopFL4_X1t/view?usp=drivesdk">https://drive.google.com/file/d/1FSKSZpPxg2wVF0yYw9wnl_KopFL4_X1t/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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