Somatic embryogenesis in Carica papaya through zygotic embryo derived callus culture (Record no. 51091)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160200.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16926
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Somatic embryogenesis in Carica papaya through zygotic embryo derived callus culture
490 0# - SERIES STATEMENT
Volume/sequential designation Acta Horticulturae, 851, p.201-208, 2010
520 3# - SUMMARY, ETC.
Summary, etc. A rapid and efficient system for papaya (Carica papaya L. cv. Co7)regeneration through indirect somatic embryogenesis has been established. Embryogenic calli were obtained from zygotic embryos (excised from 110-120 dayold fruits)on CIM [half-strength MS (Murashige and Skoog, 1962)basal salts and vitamins, 400 mg/L of glutamine, 6 percent sucrose, 4 g/L phytagel]supplemented with varying concentrations 0.5-12.0 mg/L of 2,4-D. The frequency of somatic embryo formation was as high as 54.35 percent when cultured on medium containing 2.0 mg/L of 2,4-D. Suspension cultures were superior to static cultures (solid medium)for the maturation of somatic embryos. Matured (cotyledonary stage)somatic embryos cultured on MSR medium supplemented with 0.4 mg/L BAP and 0.02 mg/L NAA showed regeneration efficiency up to 62.32 percent. The emerging shoots with 2-3 trilobed leaves were rooted on MSL medium containing 1.0 mg/L of IBA with efficiency of 60.07 percent. Plants with well-developed shoots and roots were subsequently hardened on a potting mixture enriched with arbuscular mycorrhiza. The plantlets from zygotic embryo explants were morphologically similar and normal. No phenotypic variations have been observed in field-established plants. This regeneration protocol assures a high frequency of somatic embryogenesis, maturation and regeneration into plantlets.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PAPAYA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ZYGOTIC EMBRYOS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ramaswamy, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Phap, P.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Soorianathasundaram, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kumar, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Thirugnanakumar, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sudhakar, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Balasubramanian, P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1IzO75rjKI3GzqcSJQTI886nqmTQtFE88/view?usp=drivesdk">https://drive.google.com/file/d/1IzO75rjKI3GzqcSJQTI886nqmTQtFE88/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16926 25.06.2025 25.06.2025 Documentos solicitados