Promotion of vegetative growth in force-ventilated Protea cynaroides L. explants cultured in modified temporary immersion culture vessels (Record no. 51110)
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| 000 -LEADER | |
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| fixed length control field | 03124nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160201.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16945 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Promotion of vegetative growth in force-ventilated Protea cynaroides L. explants cultured in modified temporary immersion culture vessels |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | HortScience, 53(2), p.231-235, 2018 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Temporary immersion culture vessels were modified to culture Protea cynaroides L. microshoots on semisolid growth medium. The effects of different ventilation treatments and sucrose concentrations on the vegetative growth and physiological characteristics of P. cynaroides L. microshoots were investigated. Three ventilation treatments were used: microshoots were either ventilated naturally or forced ventilated for 2 minutes/2 hours or 2 minutes/4 hours. In addition, two sucrose concentrations were used in the growth medium: 30 and 10 g·L-1. Significant interaction effects were found between ventilation and sucrose in the number of shoots formed. When cultured on growth medium with 10 g·L-1 sucrose, microshoots force-ventilated for 2 minutes/2 hours produced significantly higher number of shoots than those naturally ventilated or force-ventilated for 2 minutes/4 hours. In the 30 g·L-1 sucrose treatment, no significant differences in shoot numbers were observed among all ventilation treatments. The highest leaf areas were found in microshoots cultured in the 2 minutes/4 hours forced ventilation treatment, which were significantly higher than microshoots in the other ventilation treatments, irrespective of the sucrose concentration. Chlorophyll content was significantly higher in leaves of microshoots that were cultured in 30 g·L-1 sucrose compared with those grown in 10 g·L-1 sucrose in all ventilation treatments. Analysis of chlorophyll fluorescence of the leaves revealed that the Fv/Fm value of microshoots grown on 30 g·L-1 sucrose and force-ventilated for 2 minutes/4 hours was significantly higher than those naturally ventilated in the same sucrose treatment. Overall, the use of 30 g·L-1 sucrose in combination with 2 minutes/4 hours ventilation provided the best conditions for culturing P. cynaroides microshoots. This study demonstrated that these modified temporary immersion culture vessels can be used as a forced ventilation system to culture P. cynaroides microshoots and promote vegetative growth as well as improve their photosynthetic characteristics. The system described here introduces a simple and novel method of converting commercially available temporary immersion systems into force ventilation systems. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHLOROPHYLL FLUORESCENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FORCED VENTILATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | KING PROTEA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NATURAL VENTILATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SUCROSE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wu, H.-C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kuo, M.-L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, C.-M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1g27ZlmwjEWH0UbaTT65USS-xOUbwtkRu/view?usp=drivesdk">https://drive.google.com/file/d/1g27ZlmwjEWH0UbaTT65USS-xOUbwtkRu/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16945 | 25.06.2025 | 25.06.2025 | Documentos solicitados |