Studies on the ultrastructure and histochemistry of plant cuticles: Isolated cuticular membrane preparations of Agave americana L. and the effects of various extraction procedures (Record no. 51136)

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fixed length control field 02692nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160201.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16971
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245 10 - TITLE STATEMENT
Title Studies on the ultrastructure and histochemistry of plant cuticles: Isolated cuticular membrane preparations of Agave americana L. and the effects of various extraction procedures
490 0# - SERIES STATEMENT
Volume/sequential designation Annals of Botany, 49(6), p.769-804, 1982
520 3# - SUMMARY, ETC.
Summary, etc. The cuticular membrane (CM)of Agave americana with the adhering cellin wall was isolated with ammonium oxalate-oxalic acid solution, air-dried and dry-embedded without fixation. After KMnO4 staining, electron translucent lamellae are visible in the cuticle proper and cuticular layer. The fine structure of the opaque lamellae in the cuticle proper is more complex than previously observed in situ. It is more clearly observed in CM isolated at 40 °C than in those isolated at 100 °C, or in air-dried tissue, subsequently remoistened, fixed and dehydrated in acetone.Although extraction of CM with hot organic solvents removes substantial quantities of wax (mainly long chain alcohols and fatty acids), not all of the electron-lucent lamellae disappear completely. Strong sulphuric acid dissolves the cellin walls adhering to the CM and strongly diminishes the iodine/potassium iodide-sulphuric acid-silver proteinate staining reactivity of the CM, probably due to the marked reduction in epoxide content of the cutin. The acid does not completely remove the carbohydrate reticulum included in the cuticular layer.In sodium methoxide solution the CM is decutinized from the cellin wall side where the carbohydrate fibrillae included in the interior cuticular layer become completely exposed. On the outside, the lamellate cuticle proper is also lost. Major cutin monomers solubilized are 9, 10-epoxy-18-hydroxyoctadecanoic and 9, 10, 18-trihy-droxyoctadecanoic acids. Partial decutinization of the CM with methanolic HC1 produces similar but less drastic effects than methoxide apparently because the outer surface is protected by an artificial layer of lipids originating from depolymerized cutin.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGAVE AMERICANA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CUTICULAR MEMBRANE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CUTIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EPOXIDE GROUPS IN BIOPOLYMERS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ISOLATION OF CUTICULAR MEMBRANES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LEAF
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ULTRAHISTOCHEMISTRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element WAX
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wattendorff, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Holloway, P.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16jD52AqwVQmfllQ58MEvXI0bUejKB2db/view?usp=drivesdk">https://drive.google.com/file/d/16jD52AqwVQmfllQ58MEvXI0bUejKB2db/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16971 25.06.2025 25.06.2025 Documentos solicitados