Studies on the ultrastructure and histochemistry of plant cuticles: The cuticular membrane of Agave americana L. In situ (Record no. 51145)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160201.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16980
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245 10 - TITLE STATEMENT
Title Studies on the ultrastructure and histochemistry of plant cuticles: The cuticular membrane of Agave americana L. In situ
490 0# - SERIES STATEMENT
Volume/sequential designation Annals of Botany, 46(1), p.13-28, 1980
520 3# - SUMMARY, ETC.
Summary, etc. The outer epidermal wall of Agave americana leaves was examined in order to gain more information about the location and chemical constitution of the structural components. In middle aged leaves the wall comprised six layers which were designated epicuticular wax, cuticle proper, exterior and interior cuticular layer, exterior and interior cellin wall. A lamellated structure, consisting of a series of electron translucent lamellae of uniform thickness alternating with opaque ones of variable thickness, was observed in the thin cuticle proper on the outside of the cuticular membrane, even without heavy metal treatment. The cuticular layers underneath formed the bulk of the cuticular membrane and they also had two components, an amorphous matrix permeated by a reticulum of fibrillae.Cutin, detected with osmium and with iodine/iodine-sulphuric acid-silver proteinate, was a major component of the opaque lamellae of the cuticle proper and the matrix of the cuticular layer. Carbohydrates were absent from the cuticle proper but were detected specifically in the fibrillae of the cuticular layer and in the cellin wall. Pectic material seemed to be present on both sides of the junction between cuticular membrane and cellin wall, but no discrete zone corresponding to light microscopical observations was detected in the electron microscope. Although the lucent lamellae of the cuticle proper were tentatively ascribed to wax there was no structural or ultrahistochemical evidence for the wax component of the cuticular layer. The various ultrahistochemical reactions are discussed in relation to the known chemical composition of the membrane.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGAVE AMERICANA L.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CUTICLE PROPER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CUTICULAR LAYER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CUTICULAR MEMBRANE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EPIDERMIS WALL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ULTRAHISTOCHEMISTRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element WAX
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wattendorff, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Holloway, P.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1nNYMeqcOmn-7Ih3Pk9LqBt54xVo6A3dz/view?usp=drivesdk">https://drive.google.com/file/d/1nNYMeqcOmn-7Ih3Pk9LqBt54xVo6A3dz/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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