AFLP-based transcript profiling (cDNA-AFLP)for genome-wide expression analysis (Record no. 51201)

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control field 20250625160202.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17038
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Title AFLP-based transcript profiling (cDNA-AFLP)for genome-wide expression analysis
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Volume/sequential designation Nature Protocols, 2(6), p.1399, 2007
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Summary, etc. Although DNA microarrays are currently the standard tool for genome-wide expression analysis, their application is limited to organisms for which the complete genome sequence or large collections of known transcript sequences are available. Here, we describe a protocol for cDNA-AFLP, an AFLP-based transcript profiling method that allows genome-wide expression analysis in any species without the need for prior sequence knowledge. In essence, the cDNA-AFLP method involves reverse transcription of mRNA into double-stranded cDNA, followed by restriction digestion, ligation of specific adapters and fractionation of this mixture of cDNA fragments into smaller subsets by selective PCR amplification. The resulting cDNA-AFLP fragments are separated on high-resolution gels, and visualization of cDNA-AFLP fingerprints is described using either a conventional autoradiography platform or an automated LI-COR system. Observed differences in band intensities between samples provide a good measure of the relative differences in the gene expression levels. Identification of differentially expressed genes can be accomplished by purifying cDNA-AFLP fragments from sequence gels and subsequent sequencing. This method has found widespread use as an attractive technology for gene discovery on the basis of fragment detection and for temporal quantitative gene expression analysis. The protocol can be completed in 3-4 d.
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Topical term or geographic name entry element COMPLEMENTARY DNA
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Topical term or geographic name entry element DNA FRAGMENT
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Topical term or geographic name entry element DOUBLE STRANDED DNA
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Personal name Vuylsteke, M.
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Personal name Peleman, J. D.
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Personal name Van Eijk, M. J.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1hhcusI6hWKCCq-Tu-ROlUou1zzBQbJgC/view?usp=drivesdk">https://drive.google.com/file/d/1hhcusI6hWKCCq-Tu-ROlUou1zzBQbJgC/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17038 25.06.2025 25.06.2025 Documentos solicitados