Determination of neuraminidase kinetic constants using whole influenza virus preparations and correction for spectroscopic interference by a fluorogenic substrate (Record no. 51222)
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| fixed length control field | 02546nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160203.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17059 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Determination of neuraminidase kinetic constants using whole influenza virus preparations and correction for spectroscopic interference by a fluorogenic substrate |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | PLoS One, 8(8), p.e71401, 2013 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The influenza neuraminidase (NA)enzyme cleaves terminal sialic acid residues from cellular receptors, a process required for the release of newly synthesized virions. A balance of NA activity with sialic acid binding affinity of hemagglutinin (HA)is important for optimal virus replication. NA sequence evolution through genetic shift and drift contributes to the continuous modulation of influenza virus fitness and pathogenicity. A simple and reliable method for the determination of kinetic parameters of NA activity could add significant value to global influenza surveillance and provide parameters for the projection of fitness and pathogenicity of emerging virus variants. The use of fluorogenic substrate 2?-(4-methylumbelliferyl)-?-D-N-acetylneuraminic acid (MUNANA)and cell- or egg-grown whole influenza virus preparations have been attractive components of NA enzyme activity investigations. We describe important criteria to be addressed when determining Km and Vmax kinetic parameters using this method: (1)determination of the dynamic range of MUNANA and 4-methylumbelliferone product (4-MU)fluorescence for the instrument used; (2)adjustment of reaction conditions to approximate initial rate conditions, i.e. ?15 percent of substrate converted during the reaction, with signal-to-noise ratio ?10; (3)correction for optical interference and inner filter effect caused by increasing concentrations of MUNANA substrate. The results indicate a significant interference of MUNANA with 4-MU fluorescence determination. The criteria proposed enable an improved rapid estimation of NA kinetic parameters and facilitate comparison of data between laboratories. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CELL RECEPTOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HEMAGGLUTININ |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HYMECROMONE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marathe, B. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Leveque, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Klumpp, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Webster, R. G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Govorkova, E. A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1KBVvSyuFhW5-KDQ8x-db42bcTPh5DjM2/view?usp=drivesdk">https://drive.google.com/file/d/1KBVvSyuFhW5-KDQ8x-db42bcTPh5DjM2/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17059 | 25.06.2025 | 25.06.2025 | Documentos solicitados |