Identification of the antifungal activity of Trichoderma longibrachiatum T6 and assessment of bioactive substances in controlling phytopathgens (Record no. 51263)

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fixed length control field 03187nam a2200253Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160204.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17100
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245 10 - TITLE STATEMENT
Title Identification of the antifungal activity of Trichoderma longibrachiatum T6 and assessment of bioactive substances in controlling phytopathgens
490 0# - SERIES STATEMENT
Volume/sequential designation Pesticide BioChemistry and Physiology, 147, p.59-66, 2018
520 3# - SUMMARY, ETC.
Summary, etc. Biological control with microbial antagonists is considered an alternative approach for controlling plant diseases. Trichoderma species are one of the potential fungal biocontrol Biological control with microbial antagonists is considered an alternative approach for controlling plant diseases. Trichoderma species are one of the potential fungal biocontrol agents in suppression of soil-borne pathogens. However, the mechanism and characterization of Trichoderma spp. in inhibiting different phytopathogenic fungi are largely unknown. In this study, we investigated the antagonistic potential of the endophytic fungus Trichoderma longibrachiatum T6 as a biocontrol agent against different phytopathogenic fungi and the associated antagonistic mechanism with bioactive substances. We found that the fermentation and crude extract of T. longibrachiatum T6 had a broad spectrum and potent activity inhibiting the growth of eleven phytopathogens evaluated, and of which, the inhibitory rate against Valsa mali reached 95 percent at 5?days after incubation. Ten fractions and six sub-fractions of bioactive substances were obtained on silica gel G chromatography and Sephadex LH-20 columns. One of the sub-fractions (coded sub-Fr.4f)exhibited highest inhibition against the pathogen V. mail, with the inhibitory rate of 80.64 percent at Day 5 of the treatment. Four key chemical inhibitors were identified: (i)1, 2-Benzenedicarboxylicacid, bis (2-methylpropyl)ester (DIBP)(C16H22O4); (ii)(Z)-octadec-9-enoic acid (C18H34O2); (iii)1, 2-Benzenedicarboxylic acid, mono (2-ethylhexyl)ester (MEHP)(C16H22O4); and (iv)(Z)-13-Docosenamide (C22H43NO), using spectroscopic and nuclear magnetic resonance data. Two fungicidal compounds DIBP and MEHP provided significantly greater antifungal activities than the other compounds in the inhibition of the V. mail growth. There was a significant linear relationship between the monomer compounds MEPH or DIBP and the inhibitory rates of V. mail; at the concentration of 200??g?mL?1, the inhibitory rate reached over 86 percent or 78 percent. We conclude that the strain of T. longibrachiatum T6 can serve as an effective biocontrol agent against V. mali and the mechanism for this function was due to the secondary metabolites with effective bioactive substance.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRICHODERMA SPP.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTIFUNGAL ACTIVITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHYTOPATHOGENIC FUNGI
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOACTIVE SUBSTANCES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xu, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gan, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1qAguqlqctnhAJysyoTWKJ6qZJgrnPc3a/view?usp=drivesdk">https://drive.google.com/file/d/1qAguqlqctnhAJysyoTWKJ6qZJgrnPc3a/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17100 25.06.2025 25.06.2025 Documentos solicitados