Genome editing of upstream open reading frames enables translational control in plants (Record no. 51354)
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| fixed length control field | 01869nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160206.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17191 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Genome editing of upstream open reading frames enables translational control in plants |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Biotechnology, 36(9), p.894-900, 2018 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Translational regulation by upstream open reading frames (uORFs)is becoming established as a general mechanism for controlling the amount of protein that is synthesized from downstream primary ORFs (pORFs)1-5. We found that genome editing of endogenous uORFs in plants enabled the modulation of translation of mRNAs from four pORFs that are involved in either development or antioxidant biosynthesis. A single-guide RNA that targeted the region harboring a uORF initiation codon can produce multiple mutations. Following uORF editing, we observed varying amounts of mRNA translation in four pORFs. Notably, editing the uORF of LsGGP2, which encodes a key enzyme in vitamin C biosynthesis in lettuce, not only increased oxidation stress tolerance, but also increased ascorbate content by ~150 percent. These data indicate that editing plant uORFs provides a generalizable, efficient method for manipulating translation of mRNA that could be applied to dissect biological mechanisms and improve crops. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIOCHEMISTRY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | C (PROGRAMMING LANGUAGE) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NUCLEIC ACIDS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Si, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ji, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fan, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gao, C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/16pBoCT-1TjwHhUqhqCuS1unuVpko8S_6/view?usp=drivesdk">https://drive.google.com/file/d/16pBoCT-1TjwHhUqhqCuS1unuVpko8S_6/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17191 | 25.06.2025 | 25.06.2025 | Documentos solicitados |