Genetic transformation of banana embryogenic cells through particle bombardment using a herbicide resistance gene as selectable marker (Record no. 51490)
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| 000 -LEADER | |
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| fixed length control field | 02500nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160208.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17327 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Genetic transformation of banana embryogenic cells through particle bombardment using a herbicide resistance gene as selectable marker |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Acta Horticulturae, 575, p.61-67, 2002 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Bananas and plantains (Musa spp.)are among the most important horticultural crops in tropical and subtropical regions of the world. However, various diseases caused by fungi and viruses have seriously endangered the production of this species. Through genetic transformation technologies, disease resistant varieties may be produced. However, an effective selection process is needed to increase the efficiency of transformant recovery. For banana transformation, antibiotic resistance genes are widely used to enable this selection. The purpose of this study was to develop a simple and routine particlebombardment system to generate transgenic bananas using a herbicide resistance gene as selectable marker. Preliminary experiments were conducted to evaluate the effects of the herbicide Imazapyr on the development of embryogenic cells of "Maçã" banana (Musa sp. AAB group, Silk). A dose of 1µM of Imazapyr was chosen for the transformation experiments. Embryogenic cells were bombarded with a plasmid vector compiling the ahas gene, under control of the ahas promoter from Arabidopsis thaliana. The bombarded cells were cultured with the herbicidecontaining medium for 30 days, and putative transformed plants were regenerated. PCR analyses showed that the regenerated plants contained bombarded ahas gene. Southern hybridization confirmed the integration of the transgene into the banana genome. These results will provide a new selectable marker for banana transformation, in addition to antibiotic resistance genes, and should form the foundation for the introduction of economically important traits in banana. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIOLISTIC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IMAZAPYR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MUSA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSGENIC PLANTS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Matsumoto, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Morais, L. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vianna, G. R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aragão, F. J. L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rech, E. L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1_ASgvWnQgKJswURd8XO8ucV94Mzu_ovA/view?usp=drivesdk">https://drive.google.com/file/d/1_ASgvWnQgKJswURd8XO8ucV94Mzu_ovA/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17327 | 25.06.2025 | 25.06.2025 | Documentos solicitados |