Spatial control of in vivo CRISPR-Cas9 genome editing via nanomagnets (Record no. 51506)

MARC details
000 -LEADER
fixed length control field 01942nam a2200301Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160209.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17343
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245 10 - TITLE STATEMENT
Title Spatial control of in vivo CRISPR-Cas9 genome editing via nanomagnets
490 0# - SERIES STATEMENT
Volume/sequential designation Nature Biomedical Engineering, 3(2), p.126-136, 2018
520 3# - SUMMARY, ETC.
Summary, etc. The potential of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9)-based therapeutic genome editing is hampered by difficulties in the control of the in vivo activity of CRISPR-Cas9. To minimize any genotoxicity, precise activation of CRISPR-Cas9 in the target tissue is desirable. Here, we show that, by complexing magnetic nanoparticles with recombinant baculoviral vectors (MNP-BVs), CRISPR-Cas9-mediated genome editing can be activated locally in vivo via a magnetic field. The baculoviral vector was chosen for in vivo gene delivery because of its large loading capacity and ability to locally overcome systemic inactivation by the complement system. We demonstrate that a locally applied magnetic field can enhance the cellular entry of MNP-BVs, thereby avoiding baculoviral vector inactivation and causing a transient transgene expression in the target tissue. Because baculoviral vectors are inactivated elsewhere, gene delivery and in vivo genome editing via MNP-BVs are tissue specific.
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Topical term or geographic name entry element GENE TRANSFER
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Topical term or geographic name entry element MAGNETIC FIELDS
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Topical term or geographic name entry element NANOMAGNETICS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NANOPARTICLES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VECTORS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhu, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tong, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, C. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deshmukh, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bao, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1_Oe_pkGzQM9Mypivy4lo8vLLavqFdKp0/view?usp=drivesdk">https://drive.google.com/file/d/1_Oe_pkGzQM9Mypivy4lo8vLLavqFdKp0/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17343 25.06.2025 25.06.2025 Documentos solicitados