Optimum medium for largescale culture of Tetraselmis tetrathele (Record no. 51533)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160209.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17370
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Optimum medium for largescale culture of Tetraselmis tetrathele
490 0# - SERIES STATEMENT
Volume/sequential designation Hydrobiologia, 358, p.217-222, 1997
520 3# - SUMMARY, ETC.
Summary, etc. The prasinophyte Tetraselmis tetrathele is an alga commonly used as live food for aquatic animal larvae. The alga is usually cultured in Guillard and Ryther medium (Guilliard F)or a fertilizer enriched seawater medium for the large-scale culture of Nannochloropsis. However, Guillard F is too complicated to use for large-scale culture, and some fertilizers are impure and insoluble. A new enriched seawater medium for the large-scale culture of T. tetrathele (ES-T.T.)was formulated by modifying the Guillard F medium. NaNO 3 , NaH 2 PO 4 , Fe-EDTA and MnCl 2 were selected as essential additives for the medium by systematically removing each additive of Guillard F. Results from axenic culture experiments, using an artificial seawater medium, the required amount of these additives was estimated to be, 150 mg NaNO 3 , 10 mg NaH 2 PO 4 . 2H 2 O, 15 mg Fe-EDTA and 360 µg MnCl 2 . 4H 2 O, per liter of seawater. The productive rate of T. tetrathele in ES-T.T. was higher than in a fertilized medium in a 100 liter outdoor culture experiment. In 10 liter indoor culture experiments, no significant difference was detected in production rates between ES-T.T. and Guillard F. Therefore, ES-T.T, is simple and effective to use as a large-scale culture medium for T. tetrathele.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TETRASELMIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TETRATHELE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Okauchi, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kawamura, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/17nhlXFesTqEw3pQJyJ-O0I3znhwB1WOl/view?usp=drivesdk">https://drive.google.com/file/d/17nhlXFesTqEw3pQJyJ-O0I3znhwB1WOl/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17370 25.06.2025 25.06.2025 Documentos solicitados