Advances in the Engineering of the Gene Editing Enzymes and the Genomes: Understanding and Handling the Off-Target Effects of CRISPR/Cas9 (Record no. 51550)
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| fixed length control field | 02338nam a2200349Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160209.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17387 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Advances in the Engineering of the Gene Editing Enzymes and the Genomes: Understanding and Handling the Off-Target Effects of CRISPR/Cas9 |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Biomedical Nanotechnology, 14b456-476, 2018 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | In the past decades, significant progresses have been achieved in genetic engineering of nucleases. Among the genetically engineered nucleases, zinc finger nucleases, transcription activator-like (TAL)effector nucleases, and CRIPSPR/Cas9 system form a new field of gene editing. The gene editing efficiency or targeting effect and the off-target effect are the two major determinant factors in evaluating the usefulness of a new enzyme. Engineering strategies in improving these gene editing enzymes, particularly in minimizing their off-target effects, are the focus of this paper. Examples of using these genetically engineered enzymes in genome modification are discussed in order to better understand the requirement of engineering efforts in obtaining more powerful and useful gene editing enzymes. In addition, the identification of naturally existed anti-Cas proteins has been employed in minimizing off-target effects. Considering the future application in human gene therapy, optimization of these well recognized gene editing enzymes and exploration of more novel enzymes are both required. Before people find an ideal gene editing system having virtually no off-target effect, technologies used to screen and identify off-target effects are of importance in clinical trials employing gene therapy. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENE EDITING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENOME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENGINEERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENE THERAPY |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yin Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang Q |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xiao L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Song Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cuilan Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu X |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu X |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | He N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Feng Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1FKwI_yA9uM488MifOu3sovUn3hLLPlis/view?usp=drivesdk">https://drive.google.com/file/d/1FKwI_yA9uM488MifOu3sovUn3hLLPlis/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17387 | 25.06.2025 | 25.06.2025 | Documentos solicitados |