MARC details
| 000 -LEADER |
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| fixed length control field |
02244nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160217.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-17756 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Validated high-performance thin-layer chromatography method for the determination of nicotine in tobacco (Nicotiana tabacum L.)extracts. |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
JPC-Journal of Planar Chromatography-Modern TLC. , 25(1), p. 23-29, 2012 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A new, simple, sensitive, selective, precise, and robust high-performance thin-layer chromatography (HPTLC)method was developed and validated for the determination of nicotine in tobacco extracts. The analysis of nicotine was performed on thin-layer chromatography (TLC)glass plates precoated with silica gel 60 F254 as stationary phase. Development was carried out in a horizontal chamber under sandwich configuration with the mobile phase ethyl acetate-methanol-ammonia 2.8percent (v/v)(20:10:4, v/v/v)at room temperature (25 ± 2°C). A TLC scanner was used for spectrodensitometric scanning and analysis in the absorbance mode at 263 nm. The system was found to give compact spots for nicotine (RF value of 0.20 ± 0.02). The calibration plots showed good linear relationships with r2 = 0.9998 ± 0.0001 in the concentration range 90-900 ng/spot with respect to the peak area. According to the International Conference of Harmonization (ICH)guidelines, the TLC method was validated for precision, recovery, robustness, and ruggedness. The detection and quantification limits were determined. Statistical analysis of the data revealed that the method is reproducible and selective for the determination of nicotine content in tobacco extracts. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HIGH-PERFORMANCE THIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LAYER CHROMATOGRAPHY (HPTLC) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TOBACCO EXTRACTS, |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NICOTINE QUANTIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
METHOD VALIDATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Paillat, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Périchet, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lavoine, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Meierhenrich, U. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Fernandez, X. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1WqwHzo_huKOFg-0hkqadDukKUgyZnD4P/view?usp=drivesdk">https://drive.google.com/file/d/1WqwHzo_huKOFg-0hkqadDukKUgyZnD4P/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
