Comparative proteomic analysis of Lithospermum erythrorhizon reveals regulation of a variety of metabolic enzymes leading to comprehensive understanding of the shikonin biosynthetic pathway. (Record no. 51927)
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| fixed length control field | 02663nam a2200265Ia 4500 |
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| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160217.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17765 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Comparative proteomic analysis of Lithospermum erythrorhizon reveals regulation of a variety of metabolic enzymes leading to comprehensive understanding of the shikonin biosynthetic pathway. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant & Cell Physiology, 60(1), p.19-28, 2018 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Plants produce a large variety of specialized (secondary)metabolites having a wide range of hydrophobicity. Shikonin, a red naphthoquinone pigment, is a highly hydrophobic metabolite produced in the roots of Lithospermum erythrorhizon, a medicinal plant in the family Boraginaceae. The shikonin molecule is formed by the coupling of p-hydroxybenzoic acid and geranyl diphosphate, catalyzed by a membrane-bound geranyltransferase LePGT at the endoplasmic reticulum, followed by cyclization of the geranyl chain and oxidations; the latter half of this biosynthetic pathway, however, has not yet been clarified. To shed light on these steps, a proteome analysis was conducted. Shikonin production in vitro was specifically regulated by illumination and by the difference in media used to culture cells and hairy roots. In intact plants, however, shikonin is produced exclusively in the root bark of L. erythrorhizon. These features were utilized for comparative transcriptome and proteome analyses. As the genome sequence is not known for this medicinal plant, sequences from de novo RNA-seq data with 95,861 contigs were used as reference for proteome analysis. Because shikonin biosynthesis requires copper ions and is sensitive to blue light, this methodology identified strong candidates for enzymes involved in shikonin biosynthesis, such as polyphenol oxidase, cannabidiolic acid synthase-like and neomenthol dehydrogenase-like proteins. Because acetylshikonin is the main end product of shikonin derivatives, an O-acetyltransferase was also identified. This enzyme may be responsible for end product formation in these plant species. Taken together, these findings suggest a putative pathway for shikonin biosynthesis. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Takanashi, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nakagawa, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aburaya, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kaminade, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aoki, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saida-Munakata, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Akifumi S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Akifumi S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yazaki, K. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1aBJT9bNrjHmL7rTVP8zyjTIP-hIOERwy/view?usp=drivesdk">https://drive.google.com/file/d/1aBJT9bNrjHmL7rTVP8zyjTIP-hIOERwy/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17765 | 25.06.2025 | 25.06.2025 | Documentos solicitados |