Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China. (Record no. 51950)
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| fixed length control field | 03398nam a2200337Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160217.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17789 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Disease, 103(6), p.1213-1219, 2019 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | To provide a high-throughput, efficient, and accurate method to monitor multiple-fungicide resistance of Botrytis cinerea in the field, we used the suspension array, sequencing, and mycelial growth assay in our research. Discriminating-dose bioassays for detecting carbendazim, diethofencarb, boscalid, and iprodione resistance (CarR, DieR, BosR, and IprR, respectively)were used to analyze 257 isolates collected from Hebei Province in China during 2016 and 2017. High resistance frequencies to carbendazim (100percent), diethofencarb (92.08percent), and iprodione (86.59percent)were detected. BosR isolates accounted for 11.67percent of the total. In addition, 103 isolates were randomly selected for phenotype and genotype detection. The high-throughput suspension array was utilized to detect eight genotypes simultaneously, including BenA-E198, BenA-198A, SdhB-H272, SdhB-272Y, BcOS1-I365, BcOS1-365S, erg27-F412, and erg27-412S, which were associated with resistance toward carbendazim or diethofencarb, boscalid, iprodione, and fenhexamid (FenR), respectively. Most of the benzimidazole-resistant isolates (81.55percent)possessed the E198V mutation in the BenA gene. Ninety-three isolates with dual resistance to carbendazim and diethofencarb showed the E198V/K mutation. All BosR isolates carried the H272R mutation in the SdhB gene. The I365S and Q369P+N373S (66.99percent)mutations in the BcOS1 gene were more frequently observed. No mutation was detected in the erg27 gene in Hebei isolates. There were 13 resistance profile phenotypes. Phenotypes with triple resistance were the most common (83.50percent), and CarRDieRBosSIprRFenS was the major type. CarR isolates that carried E198V/K/A were all highly resistant (HR)and only one F200Y mutant was moderately resistant (MR)to carbendazim. Isolates that possessed E198V/K were MR or HR to diethofencarb. BosR isolates that possessed H272R mutation were lowly resistant (LR). IprR isolates were all LR or MR. The distribution of half maximal effective concentrations of CarR isolates with E198V/K mutations and IprR isolates with Q369P+N373S mutations significantly increased from 2016 to 2017. Combined with our observations, a combination method of the high-throughput suspension array and the mycelial growth assay was suggested to accurately monitor multiple resistance of B. cinerea in the field. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Su, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shang, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ma, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mawulipplimi Adzavon, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mawulipplimi Adzavon, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Weng, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Han, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ma, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao, |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xie, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1x7Vpv2vaYWwJwT2-hgDIg3TL9CM_6kWJ/view?usp=drivesdk">https://drive.google.com/file/d/1x7Vpv2vaYWwJwT2-hgDIg3TL9CM_6kWJ/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17789 | 25.06.2025 | 25.06.2025 | Documentos solicitados |