Proteome Analysis Using Gel-LC-MS/MS (Record no. 51969)
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| 000 -LEADER | |
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| fixed length control field | 02138nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160217.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17808 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Proteome Analysis Using Gel-LC-MS/MS |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Current Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.93, 2019 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | This article describes processing of protein samples using 1D SDS gels prior to protease digestion for proteomics workflows that subsequently utilize reversed-phase nanocapillary ultra-high-pressure liquid chromatography (LC)coupled to tandem mass spectrometry (MS/MS). The resulting LC-MS/MS data are used to identify peptides and thereby infer proteins present in samples ranging from simple mixtures to very complex proteomes. Bottom-up proteome studies usually involve quantitative comparisons across several or many samples. For either situation, 1D SDS gels represent a simple, widely available technique that can be used to either fractionate complex proteomes or rapidly clean up low microgram samples with minimal losses. After gel separation and staining/destaining, appropriate gel slices are excised, and in-gel reduction, alkylation, and protease digestion are performed. Digests are then processed for LC-MS/MS analysis. Protocols are described for either sample fractionation with high-throughput processing of many samples or simple cleanup without fractionation. An optional strategy is to conduct in-solution reduction and alkylation prior to running gels, which is advantageous when a large number of samples will be separated into large numbers of fractions. Optimization of trypsin digestion parameters and comparison to in-solution protease digestion are also described. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Goldman, A. R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Beer, L. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tang, H. Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hembach, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zayas-Bazan, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Speicher, D. W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1IqOjZ90eMUoQNK_q7FqiP1hTxwiLISXl/view?usp=drivesdk">https://drive.google.com/file/d/1IqOjZ90eMUoQNK_q7FqiP1hTxwiLISXl/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17808 | 25.06.2025 | 25.06.2025 | Documentos solicitados |