Evaluation of molecular and genetic approaches to generate glucoamylase overproducing strains of Aspergillus niger. (Record no. 51983)
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| fixed length control field | 02611nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160218.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17822 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Evaluation of molecular and genetic approaches to generate glucoamylase overproducing strains of Aspergillus niger. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Biotechnology , 36(2), p.165-175, 1994 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | To evaluate the possibility of improving glucoamylase (GLA)production in Aspergillus niger strains carrying multiple copies of the GLA encoding gene (glaA), additional glaA copies were introduced either by genetic recombination or retransformation. For strains to be used in such experiments a genetic analysis was first carried out. The results of this analysis clearly revealed that in each transformant intergration had occurred at a chromosome corresponding to a single linkage group (LG). The GLA production per gene copy showed considerable variation in these strains, indicating a clear effect of the site of integration on gene expression. Introduction of additional gene copies by genetic recombination experiments was carried out for different combinations of strains, carrying glaA copies in different chromosomes. The introduction of additional glaA gene copies by genetic recombination did not result in a considerable increase in GLA production compared to the parental strains. In some strains recombination resulted in genetic instability, observed by the frequent loss of glaA copies. Also, retransformation of multi-copy glaA strains did not result in an increase in GLA production. In several strains even a decrease in GLA production was found after retransformation. Southern analysis of these transformants suggested that newly introduced gene copies were heavily rearranged, which partly explains why GLA production was not increased. Further analysis of one such transformant provided evidence that the overexpression of the glaA gene is limited by the amount of trans-acting regulatory protein(s)available |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENETIC STABILITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEIN OVERPRODUCTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHEF ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENE REGULATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FILAMENTOUS FUNGI |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Verdoes, J.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Diepeningen, A. D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Punt, P. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Debets, A. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Stouthamer, A. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Den Hondel, C. A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/12LVZAgMKirL76Z31jjYRlc4m2OWF7E1N/view?usp=drivesdk">https://drive.google.com/file/d/12LVZAgMKirL76Z31jjYRlc4m2OWF7E1N/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17822 | 25.06.2025 | 25.06.2025 | Documentos solicitados |