Purification and some properties of ?2-isopentenylpyrophosphate: 5? AMP ?2-isopentenyltransferase from the cellular slime mold Dictyostelium discoideum. (Record no. 52030)
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| fixed length control field | 01999nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160219.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17869 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Purification and some properties of ?2-isopentenylpyrophosphate: 5? AMP ?2-isopentenyltransferase from the cellular slime mold Dictyostelium discoideum. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Archives of BioChemistry and Biophysics, 230(2), p.652-660, 1984 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | 2-Isopentenylpyrophosphate:5?AMP ?2-isopentenyltransferase, which catalyzes the formation of isopentenyl-AMP from ?2-isopentenylpyrophosphate and 5?AMP, was purified 6800-fold from the fruiting body of the cellular slime mold Dictyostelium discoideum using several separation procedures including 5?AMPox-redAH-Sepharose 4B affinity column chromatography. The final preparation was very unstable and lost its activity in a day. Various properties of the 1000-fold-purified enzyme preparation were examined. The molecular mass was 40,000 ± 2000 Da, as determined by Sephadex G-100 superfine gel filtration. The divalent metal ions Mn2+, Zn2+, and Mg2+ profoundly affected the enzymatic activity depending on their concentration, and also altered the optimum pH and temperature. Of the compounds tested, 5?AMP was the best acceptor of the isopentenyl group and, interestingly, ADP also served as a substrate, being 60-80 per cent as effective as 5?AMP. Adenine, adenosine, and ATP were not substrates for this enzyme. Under the optimum assay conditions (pH 7.0, 1 mm Zn2+, and 25 °C)the Km values for 5?AMP and ?2-isopentenylpyrophosphate were 1.0 × 10?7m and 2.2 × 10?6m, respectively. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GERMINATION INHIBITOR |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ihara, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Taya, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nishimura, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tanaka, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/16NvHMrzUegwM_YqF7wazys48juqHxLuG/view?usp=drivesdk">https://drive.google.com/file/d/16NvHMrzUegwM_YqF7wazys48juqHxLuG/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17869 | 25.06.2025 | 25.06.2025 | Documentos solicitados |