MARC details
| 000 -LEADER |
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| fixed length control field |
02209nam a2200361Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160219.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-17898 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus. |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Molecular Cell, https://doi.org/10.1016/j.molcel.2019.08.014, 2019 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Fibrillar centers (FCs)and dense fibrillar components (DFCs)are essential morphologically distinct sub-regions of mammalian cell nucleoli for rDNA transcription and pre-rRNA processing. Here, we report that a human nucleolus consists of several dozen FC/DFC units, each containing 2-3 transcriptionally active rDNAs at the FC/DFC border. Pre-rRNA processing factors, such as fibrillarin (FBL), form 18-24 clusters that further assemble into the DFC surrounding the FC. Mechanistically, the 5? end of nascent 47S pre-rRNA binds co-transcriptionally to the RNA-binding domain of FBL. FBL diffuses to the DFC, where local self-association via its glycine- and arginine-rich (GAR)domain forms phase-separated clusters to immobilize FBL-interacting pre-rRNA, thus promoting directional traffic of nascent pre-rRNA while facilitating pre-rRNA processing and DFC formation. These results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FIBRILLARIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GLYCINE- AND ARGININE-RICH |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
INTRINSICALLY DISORDERED REGION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA BINDING DOMAIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA SORTING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NUCLEOLUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CONDENSATES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RIBOSOMAL RNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PHASE SEPARATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SUPER-RESOLUTION MICROSCOPY |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yao, R. W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xu, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Shan, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Luan, P. F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Chen, L. L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1kU9zWp_5Hl2xQ2ls5p-A9FZjotD9o3D2/view?usp=drivesdk">https://drive.google.com/file/d/1kU9zWp_5Hl2xQ2ls5p-A9FZjotD9o3D2/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
