Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions (Record no. 52115)
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| fixed length control field | 01964nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160220.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17955 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Biotechnology, 31(12), p.1119, 2013 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Genomes assembled de novo from short reads are highly fragmented relative to the finished chromosomes of Homo sapiens and key model organisms generated by the Human Genome Project. To address this problem, we need scalable, cost-effective methods to obtain assemblies with chromosome-scale contiguity. Here we show that genome-wide chromatin interaction data sets, such as those generated by Hi-C, are a rich source of long-range information for assigning, ordering and orienting genomic sequences to chromosomes, including across centromeres. To exploit this finding, we developed an algorithm that uses Hi-C data for ultra-long-range scaffolding of de novo genome assemblies. We demonstrate the approach by combining shotgun fragment and short jump mate-pair sequences with Hi-C data to generate chromosome-scale de novo assemblies of the human, mouse and Drosophila genomes, achieving-for the human genome-98 per cent accuracy in assigning scaffolds to chromosome groups and 99 per cent accuracy in ordering and orienting scaffolds within chromosome groups. Hi-C data can also be used to validate chromosomal translocations in cancer genomes. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHROMOSOME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHROMATIN INTERACTIONS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Burton, J. N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Adey, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Patwardhan, R. P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Qiu, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kitzman, J. O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shendure, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1uSjciEGEGy7K32N6_dVWKeDU-qiBKxQn/view?usp=drivesdk">https://drive.google.com/file/d/1uSjciEGEGy7K32N6_dVWKeDU-qiBKxQn/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17955 | 25.06.2025 | 25.06.2025 | Documentos solicitados |