A short history of electrophoretic methods (Record no. 52129)

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control field 20250625160221.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17969
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Title A short history of electrophoretic methods
490 0# - SERIES STATEMENT
Volume/sequential designation Electrophoresis, 14(1), p.1243-1249, 1993
520 3# - SUMMARY, ETC.
Summary, etc. High resolution separation of proteins, based on charge differences, is possible with disc electrophoresis, displacement electrophoresis (isotachophoresis)and notably isoelectric focusing (IEF). Size separation is obtained in sodium dodecyl sulfate?polyacrylamide gel electrophoresis (SDS?PAGE). The combination of gel IEF, followed by SDS?PAGE in a second?dimensional slab gel, i.e. two?dimensional gel electrophoresis, affords the highest resolution with up to several thousand spots per gel. Staining of proteins gives high resolution patterns which can be scanned and stored in comprehensive databases. Over the last 10 years the electrophoretic separation in gels and subsequent visualization of nucleic acids (DNA, RNA)and even genes as well as nucleotides have been much improved, making possible efficient mapping of the genes in humans and all other organisms. This has led to the biggest concerted endeavor in the history of science, i.e. the mapping of the human genome, which will be of importance as long as mankind exists. In the last years electrophoresis in capillaries has attracted much interest because for numerous substances, such as proteins nucleic acids, pharmaceuticals, metabolites, and peptides, it offers high resolution on the analytical scale with over 1 million theoretical plates. Electrophoretic methods have unprecedented impact on life sciences, providing a basis for unique advances in biochemistry, molecular biology, genetics, gene technology and medicine.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ELECTROPHORETIC
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vesterberg, O.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1XlT08LVQCcPt9Et4_XEi9lNC0eVPAPpU/view?usp=drivesdk">https://drive.google.com/file/d/1XlT08LVQCcPt9Et4_XEi9lNC0eVPAPpU/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17969 25.06.2025 25.06.2025 Documentos solicitados