Recurrent somatic embryogenesis and development of somatic embryos in Akebia trifoliata (Thunb.)Koidz (Lardizabalaceae) (Record no. 52130)
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| 000 -LEADER | |
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| fixed length control field | 02600nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160221.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-17970 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Recurrent somatic embryogenesis and development of somatic embryos in Akebia trifoliata (Thunb.)Koidz (Lardizabalaceae) |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Cell, Tissue and Organ Culture , p.1-12, 2019 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A simple and effective protocol was established for recurrent somatic embryogenesis and plant regeneration in Akebia trifoliata (Thunb.)Koidz. Somatic embryos directly formed from the root zone of the immature zygotic embryos cultured on MS medium devoid of plant growth regulator (PGR). The induction frequency of immature zygotic embryos was 52.5 per cent, and the mean number of somatic embryos reached up to 19.5. Secondary somatic embryos arose on the primary embryos at a high frequency (95.8 per cent), and this process maintained in a recurrent way on PGR-free MS medium. The highest mean number of somatic embryos also appeared in the primary embryos which can reach up to 44.7. While both of the embryogenic potential and mean number of embryos per explant displayed a gradual diminution with subculturing. The addition of 0.5 mg l?1 6-benzyladenine in the 1/2 MS regeneration medium can significantly improve the frequency of somatic embryos convert to plantlets. The microscopic analysis revealed that the development process of somatic embryos via the globular, heart, torpedo and cotyledonal stages in A. trifoliata, the histological analysis showed that somatic embryos directly initiated from root epidermal cells and there are no vascular connections between the somatic embryos and maternal tissue. Regenerated plantlets acclimated successfully to greenhouse conditions. Approximately 65 per cent plantlets survived and displayed no morphological characteristics differences with seed-derived plants. The simple and effective protocols established in this study will promote large-scale clonal propagation and genetic improvement of A. trifoliata. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AKEBIA TRIFOLIATA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DEVELOPMENT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HORMONE-FREE MEDIUM |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zou, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yao, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhong, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Huang, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1vlyT8iU_0wdUvqGOCxFq-Cux3sgJB9sz/view?usp=drivesdk">https://drive.google.com/file/d/1vlyT8iU_0wdUvqGOCxFq-Cux3sgJB9sz/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-17970 | 25.06.2025 | 25.06.2025 | Documentos solicitados |