Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice (Record no. 52136)

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fixed length control field 02946nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160221.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17977
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245 10 - TITLE STATEMENT
Title Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice
490 0# - SERIES STATEMENT
Volume/sequential designation Planta, 249(3), p.891-912, 2019
520 3# - SUMMARY, ETC.
Summary, etc. Expression of the Galactinol synthase genes in rice is regulated through post-transcriptional intron retention in response to abiotic stress and may be linked to Raffinose Family Oligosaccharide synthesis in osmotic perturbation. Galactinol synthase (GolS)is the first committed enzyme in raffinose family oligosaccharide (RFO)synthesis pathway and synthesizes galactinol from UDP-galactose and inositol. Expression of GolS genes has long been implicated in abiotic stress, especially drought and salinity. A non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes was identified in rice photosynthetic tissue. We found that the two isoforms of Oryza sativa GolS (OsGolS)gene, located in chromosomes 3(OsGolS1)and 7(OsGolS2)are interspersed by conserved introns harboring characteristic premature termination codons (PTC). During abiotic stress, the premature and mature transcripts of both isoforms were found to accumulate in a rhythmic manner for very small time-windows interrupted by phases of complete absence. Reporter gene assay using GolS promoters under abiotic stress does not reflect this accumulation profile, suggesting that this regulation occurs post-transcriptionally. We suggest that this may be due to a surveillance mechanism triggering the degradation of the premature transcript preventing its accumulation in the cell. The suggested mechanism fits the paradigm of PTC-induced Nonsense-Mediated Decay (NMD). In support of our hypothesis, when we pharmacologically blocked NMD, the full-length pre-mRNAs were increasingly accumulated in cell. To this end, our work suggests that a combined transcriptional and post transcriptional control exists in rice to regulate GolS expression under stress. Concurrent detection and processing of prematurely terminating transcripts coupled to repressed splicing can be described as a form of Regulated Unproductive Splicing and Translation (RUST)and may be linked to the stress adaptation of the plant, which is an interesting future research possibility.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RICE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GOLS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ABIOTIC STRESS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RUST
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SPLICING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REGULATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mukherjee, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sengupta, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mukherjee, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Basak, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Majumder, A. L.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1y_k7XD7qbnDoiRcYM-R_19ARPR6pyCKa/view?usp=drivesdk">https://drive.google.com/file/d/1y_k7XD7qbnDoiRcYM-R_19ARPR6pyCKa/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17977 25.06.2025 25.06.2025 Documentos solicitados