Functional divergence of PIN1 paralogous genes in rice. (Record no. 52245)

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fixed length control field 02239nam a2200265Ia 4500
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control field MX-MdCICY
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control field 20250625160223.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18087
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245 10 - TITLE STATEMENT
Title Functional divergence of PIN1 paralogous genes in rice.
490 0# - SERIES STATEMENT
Volume/sequential designation Plant & Cell Physiology, 60(12), p.2720-2732, 2019
520 3# - SUMMARY, ETC.
Summary, etc. Auxin is a phytohormone that plays an important role in plant growth and development by forming local concentration gradients. The regulation of auxin levels is determined by the activity of auxin efflux carrier protein PIN-formed (PIN). In Arabidopsis thaliana, PIN-formed1 (PIN1)functions in inflorescence and root development. In rice (Oryza sativa L.), there are four PIN1 homologs (OsPIN1a-1d), but their functions remain largely unexplored. Hence, in this study, we created mutant alleles of PIN1 gene-pin1a, pin1b, pin1c, pin1d, pin1a pin1b and pin1c pin1d- using CRISPR/Cas9 technology and used them to study the functions of the four OsPIN1 paralogs in rice. In wild-type rice, all four OsPIN1 genes were relatively highly expressed in the root than in other tissues. Compared with the wild type, the OsPIN1 single mutants had no dramatic phenotypes, but the pin1a pin1b double mutant had shorter shoots and primary roots, fewer crown roots, reduced root gravitropism, longer root hairs and larger panicle branch angle. Furthermore, the pin1c pin1d double mutant showed no observable phenotype at the seedling stage, but showed naked, pin-shape inflorescence at flowering. These data suggest that OsPIN1a and OsPIN1b are involved in root, shoot and inflorescence development in rice, whereas OsPIN1c and OsPIN1d mainly function in panicle formation. Our study provides basic knowledge that will facilitate the study of auxin transport and signaling in rice.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRISPR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ADAPTATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRISPR-CAS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhu, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wu, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shao, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wu, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mao, C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1YHlh_ayQIuw9GfEYsG311NmX5m5pzOTV/view?usp=drivesdk">https://drive.google.com/file/d/1YHlh_ayQIuw9GfEYsG311NmX5m5pzOTV/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18087 25.06.2025 25.06.2025 Documentos solicitados