Developmental gene regulation in Aspergillus nidulans (Record no. 52273)

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control field 20250625160223.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18115
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Title Developmental gene regulation in Aspergillus nidulans
490 0# - SERIES STATEMENT
Volume/sequential designation Developmental Biology, 78(2), p.497-510, 1980
520 3# - SUMMARY, ETC.
Summary, etc. The Ascomycete fungus Aspergillus nidulans reproduces asexually by differentiating conidiophores and conidia. Gene regulation during asexual reproduction was investigated by comparing poly(A)RNA populations derived from somatic hyphae, conidiating cultures and purified conidia. Single-copy and complementary DNA hybridization experiments showed that vegetative cells contained 5600-6000 diverse, average-sized poly(A)RNA sequences distributed into three prevalence classes. cDNA hybridization experiments indicated that a significant proportion of the poly(A)RNA derived from either conidiating cultures or spores consisted of sequences absent from somatic hyphae. To assess accurately the degree to which the poly(A)RNA populations differed, cDNA preparations were isolated which were complementary to sequences present only in conidia or in conidiating cultures. Hybridization of these cDNAs with poly(A)RNA from conidiating cultures showed that approximately 18.5 per cent of the poly(A)RNA mass comprised 1300 diverse sequences not present in somatic cells. Of these, about 300 were present only in conidia. The remainder were accumulated specifically during sporulation, but were absent from spores. Analogous experiments showed that the great majority of the poly(A)RNA sequences accumulated by vegetative hyphae were also present in conidiating cultures. Thus, cell differentiation during A. nidulans asexual reproduction involves the accumulation of many new poly(A)RNA sequences, but not the loss of preexisting ones.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASPERGILLUS NIDULANS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Timberlake, W. E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1zszyfQqGusa5DgL7BTOylimi9zW658al/view?usp=drivesdk">https://drive.google.com/file/d/1zszyfQqGusa5DgL7BTOylimi9zW658al/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18115 25.06.2025 25.06.2025 Documentos solicitados