Identification of carotenoid cleavage dioxygenases from Nostoc sp. PCC 7120 with different cleavage activities. (Record no. 52274)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18116
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245 10 - TITLE STATEMENT
Title Identification of carotenoid cleavage dioxygenases from Nostoc sp. PCC 7120 with different cleavage activities.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Biological Chemistry, 281(42), p.31583-31593, 2006
520 3# - SUMMARY, ETC.
Summary, etc. Carotenoid cleavage dioxygenases (CCDs)are a class of enzymes that oxidatively cleave carotenoids into apocarotenoids. Dioxygenases have been identified in plants and animals and produce a wide variety of cleavage products. Despite what is known about apocarotenoids in higher organisms, very little is known about apocarotenoids and CCDs in microorganisms. This study surveyed cleavage activities of ten putative carotenoid cleavage dioxygenases from five different cyanobacteria in recombinant Escherichia coli cells producing different carotenoid substrates. Three CCD homologs identified in Nostoc sp. PCC 7120 were purified, and their cleavage activities were investigated. Two of the three enzymes showed cleavage of ?,?-carotene at the 9,10 and 15,15? positions, respectively. The third enzyme did not cleave full-length carotenoids but cleaved the apocarotenoid ?-apo-8?-carotenal at the 9,10 position. 9,10-Apocarotenoid cleavage specificity has previously not been described. The diversity of carotenoid cleavage activities identified in one cyanobacteria suggests that CCDs not only facilitate the degradation of photosynthetic pigments but generate apocarotenals with yet to be determined biological roles in microorganisms.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAROTENOID CLEAVAGE DIOXYGENASES (CCDS)
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marasco, E. K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vay, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schmidt-Dannert, C.
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18116 25.06.2025 25.06.2025 Documentos solicitados