Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells. (Record no. 52275)

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fixed length control field 02508nam a2200313Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160223.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18117
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells.
490 0# - SERIES STATEMENT
Volume/sequential designation Plant physiology and BioChemistry, 65, p.1-8, 2013
520 3# - SUMMARY, ETC.
Summary, etc. Phosphatidic acid (PA)is the common lipid product in abscisic acid (ABA)and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid)rapidly (minutes)in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate phosphatase 1 and 2 isoforms (E.C. 3.1.3.4)was verified in isolated aleurone membranes in vitro, the former but not the latter being specifically responsive to the presence of GA or ABA. The in vitro DGPP phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase - that allows the production of 18:2-DGPP from 18:2-PA - is stimulated by ABA. The long term effects (24 h)of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the substrate of PAP1 and/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment. The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubation.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALEURONE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ABSCISIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DIACYLGLYCEROL PYROSPHOSHATE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GIBBERELLIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHATIDIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHATIDATE PHOSPHATASES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Villasuso, A. L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Di Palma, M. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Aveldaño, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pasquaré, S. J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Racagni, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Giusto, N. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Machado, E. E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1vi4mEm1PE62f_qzZgygnFHWvYwtF-DkH/view?usp=drivesdk">https://drive.google.com/file/d/1vi4mEm1PE62f_qzZgygnFHWvYwtF-DkH/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18117 25.06.2025 25.06.2025 Documentos solicitados