Auxin-Binding Protein in Etiolated Mung Bean Seedlings: Purification and Properties of Auxin-Bindmg Protein-II. (Record no. 52276)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160223.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18118
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245 10 - TITLE STATEMENT
Title Auxin-Binding Protein in Etiolated Mung Bean Seedlings: Purification and Properties of Auxin-Bindmg Protein-II.
490 0# - SERIES STATEMENT
Volume/sequential designation Plant & Cell Physiology, 26(1), p.185-192, 1985
520 3# - SUMMARY, ETC.
Summary, etc. An auxin-binding protein (ABP-II)was purified from the extract of etiolated mung bean seedlings by affinity chromatography on 2,4-D-linked Sepharose 4B and by gel filtration on Sepharose 4B and Sephacryl S-200. The molecular weight was estimated to be about 190,000 by gel filtration on Sephacryl S-200. ABP-II gave a single band corresponding to a molecular weight of about 48,000 on SDS-polyacrylamide gel electrophoresis. The dissociation constants of ABP-II for 2,4-D determined by amrnonium sulfate precipitation and equilibrium dialysis were 9.5×10?6 M and 1.1×10?5 M, respectively. 14C-2,4-D-binding to ABP-II was reversible and inhibited by addition of IAA, naphthalene-1-acetic acid, 2,4,5-trichlorophenoxyacetic acid or p-chlorophenoxyisobutylic acid to the assay mixture.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AFFINITY CHROMATOGRAPHY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AUXIN-BINDING PROTEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 2,4-DICHLOROPHENOXYACETIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INDOLE-3-ACETIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MUNG BEAN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sakai, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1mDP4KcSA9NITmRRhJk9ANgkUvb2iTeQ_/view?usp=drivesdk">https://drive.google.com/file/d/1mDP4KcSA9NITmRRhJk9ANgkUvb2iTeQ_/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18118 25.06.2025 25.06.2025 Documentos solicitados