Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA)from Aspergillus oryzae. (Record no. 52279)

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control field 20250625160223.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18121
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Title Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA)from Aspergillus oryzae.
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Volume/sequential designation Gene, 108(1), p.145-150, 1991
520 3# - SUMMARY, ETC.
Summary, etc. The glucoamylase-encoding gene (glaA)from Aspergillus oryzae was cloned using its cDNA as a probe, which had been isolated previously. From comparison of nucleotide (nt)sequences of genomic clones with its cDNA, the glaA gene was found to contain four short putative introns, 45-56 nt in length. The A. oryzaeglaA gene shared 62 per cent homology at the nt level with the A. nigerglaA gene with the four introns located at the same position. The 5'-flanking region contained a TATA box at nt ?72 from the start codon, and two putative CAAT sequences at nt ?87 and ?331. Genomic Southern analysis and physical mapping showed that the glaA gene is located on the smallest chromosome (3.4 Mb)of six separated bands of chromosomes. Clones containing the glaA gene, when re-introduced intro A. oryzae, resulted in a three- to eightfold increase in glucoamylase activity.
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Topical term or geographic name entry element RECOMBINANT DNA
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Topical term or geographic name entry element INTRON
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Topical term or geographic name entry element TRANSFORMATION
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Topical term or geographic name entry element GENE STRUCTURE
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Topical term or geographic name entry element PHYSICAL MAPPING
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Topical term or geographic name entry element SAKE
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Topical term or geographic name entry element ASCOMYCETES
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Personal name Yoji, H.
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Personal name Kozo, T.
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Personal name Katsuhiko, K.
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Personal name Katsuya, G.
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Personal name Chieko, K.
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Personal name Gakuzo, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shodo, H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Hq0qf0V1mZ_uE-i080E0Kp4hYoyLSavO/view?usp=drivesdk">https://drive.google.com/file/d/1Hq0qf0V1mZ_uE-i080E0Kp4hYoyLSavO/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18121 25.06.2025 25.06.2025 Documentos solicitados