Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion. (Record no. 52280)

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fixed length control field 01375nam a2200253Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160223.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18122
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245 10 - TITLE STATEMENT
Title Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion.
490 0# - SERIES STATEMENT
Volume/sequential designation Bio/technology, 8(5), p.435-440, 1990
520 3# - SUMMARY, ETC.
Summary, etc. We have extended the work on chymosin production in Aspergillus by constructing an expression vector in which the cDNA encoding bovine prochymosin B was fused in frame immediately following the codon for the last amino acid of the A. awamori glucoamylase (glaA)gene. Transformation of A. awamori with this plasmid led to the secretion of considerably higher amounts of chymosin than obtained with previous chymosin expression vectors. We present evidence that mature chymosin is autocatalytically released from the glucoamylase-chymosin fusion protein after secretion.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHYMOSIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASPERGILLUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHYMOSIN FUSION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ward, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wilson, L. J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kodama, K. H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rey, M. W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Berka, R. M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1SoAfh_RwsWzY1C1V-oDQe0V90l1eNEKA/view?usp=drivesdk">https://drive.google.com/file/d/1SoAfh_RwsWzY1C1V-oDQe0V90l1eNEKA/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18122 25.06.2025 25.06.2025 Documentos solicitados