Biofabrication of human articular cartilage: a path towards the development of a clinical treatment (Record no. 52427)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02711nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160226.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-18274 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Biofabrication of human articular cartilage: a path towards the development of a clinical treatment |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biofabrication, 10(4), p.045006, 2018 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Cartilage injuries cause pain and loss of function, and if severe may result in osteoarthritis (OA). 3D bioprinting is now a tangible option for the delivery of bioscaffolds capable of regenerating the deficient cartilage tissue. Our team has developed a handheld device, the Biopen, to allow in situ additive manufacturing during surgery. Given its ability to extrude in a core/shell manner, the Biopen can preserve cell viability during the biofabrication process, and it is currently the only biofabrication tool tested as a surgical instrument in a sheep model using homologous stem cells. As a necessary step toward the development of a clinically relevant protocol, we aimed to demonstrate that our handheld extrusion device can successfully be used for the biofabrication of human cartilage. Therefore, this study is a required step for the development of a surgical treatment in human patients. In this work we specifically used human adipose derived mesenchymal stem cells (hADSCs), harvested from the infra-patellar fat pad of donor patients affected by OA, to also prove that they can be utilized as the source of cells for the future clinical application. With the Biopen, we generated bioscaffolds made of hADSCs laden in gelatin methacrylate, hyaluronic acid methacrylate and cultured in the presence of chondrogenic stimuli for eight weeks in vitro. A comprehensive characterisation including gene and protein expression analyses, immunohistology, confocal microscopy, second harmonic generation, light sheet imaging, atomic force mycroscopy and mechanical unconfined compression demonstrated that our strategy resulted in human hyaline-like cartilage formation. Our in situ biofabrication approach represents an innovation with important implications for customizing cartilage repair in patients with cartilage injuries and OA. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENGINEERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHYSICAL SCIENCES AND MATHEMATICS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Onofrillo, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Duchi, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | D O'Connell, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Blanchard, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | O'Connor, A. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Scott, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Di Bella, C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1FZxhFIMGtC-znyILA8NlmE_QkNEnL2-z/view?usp=drivesdk">https://drive.google.com/file/d/1FZxhFIMGtC-znyILA8NlmE_QkNEnL2-z/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-18274 | 25.06.2025 | 25.06.2025 | Documentos solicitados |