SDS polyacrylamide gel electrophoresis of proteins (Record no. 52475)

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control field 20250625162410.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18323
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Title SDS polyacrylamide gel electrophoresis of proteins
490 0# - SERIES STATEMENT
Volume/sequential designation In Proteins. Humana Press, p.41-55 , 1984
520 3# - SUMMARY, ETC.
Summary, etc. Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate)to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Commonly, about 1.4 g SDS is bound per 1 g protein, although there are exceptions to this rule. The proteins are generally denatured and solubilized by their binding of SDS, and the complex forms a prolate elipsoid or rod of a length roughly proportionate to the protein's molecular weight. Thus, proteins of either acidic or basic pI form negatively charged complexes that can be separated on the bases of differences in charges and sizes by electrophoresis through a sieve-like matr ix of polyacrylamide gel.
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Topical term or geographic name entry element SODIUM LAURYL SULFATE
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Topical term or geographic name entry element AMMONIUM PERSULFATE
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Topical term or geographic name entry element ACRYLAMIDE CONTENT
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Topical term or geographic name entry element ACRYLAMIDE MONOMER
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Topical term or geographic name entry element RESERVOIR BUFFER
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Smith, B. J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1j1t9b-IKX9axYf2B7-Xy-bmanPsuydpX/view?usp=drivesdk">https://drive.google.com/file/d/1j1t9b-IKX9axYf2B7-Xy-bmanPsuydpX/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18323 25.06.2025 25.06.2025 Documentos solicitados