Stability of lipases in miniemulsion systems: Correlation between secondary structure and activity. (Record no. 52500)

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fixed length control field 02225nam a2200265Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162410.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18348
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Stability of lipases in miniemulsion systems: Correlation between secondary structure and activity.
490 0# - SERIES STATEMENT
Volume/sequential designation Enzyme and microbial technology, 114, p.7-14, 2018
520 3# - SUMMARY, ETC.
Summary, etc. The exploitation of an efficient enzymatic system to perform biopolymers synthesis, namely polyesters from dicarboxylic acids and dialcohols, requires the evaluation of the enzyme operational stability. This becomes particularly relevant when non-conventional media, such as miniemulsions, are used due to the inhomogeneity of the reaction media and presence of surfactants and high concentrations of organic compounds which might be deleterious to the structure-function of the enzyme. The stability of three lipases, Candida sp., Candida rugosa and Burkholderia cepacia, in miniemulsions during polyester synthesis, was accessed through the secondary structure integrities and activities in order to establish any putative correlations between secondary structure and activity. The effect of the individual components that constitute the emulsion system was also evaluated to identify those which are more disruptive to the secondary structure-function of the enzyme. Depending on the lipase and the presence of different reaction components, three scenarios were observed: a close correlation between secondary structural changes and activity, a drop in activity with no secondary structure alterations but unfolding of tertiary structure and disruption of secondary structure that allows regain of activity in the presence of substrate.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENZYME STABILITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPASES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYESTER SYNTHESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MINIEMULSIONS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FAR-UV CIRCULAR DICHROISM
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pfluck, A. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Barros, D. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fonseca, L. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Melo, E. P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1zYBTKtHEKhdUdkJZypRV19hd9rd6AuEj/view?usp=drivesdk">https://drive.google.com/file/d/1zYBTKtHEKhdUdkJZypRV19hd9rd6AuEj/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18348 25.06.2025 25.06.2025 Documentos solicitados