Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps. (Record no. 52549)

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control field MX-MdCICY
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control field 20250625162411.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18397
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Title Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps.
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Volume/sequential designation Nature Plants, 4(11), p.879-887, 2018
520 3# - SUMMARY, ETC.
Summary, etc. Plant genomes are often characterized by a high level of repetitiveness and polyploid nature. Consequently, creating genome assemblies for plant genomes is challenging. The introduction of short-read technologies 10 years ago substantially increased the number of available plant genomes. Generally, these assemblies are incomplete and fragmented, and only a few are at the chromosome scale. Recently, Pacific Biosciences and Oxford Nanopore sequencing technologies were commercialized that can sequence long DNA fragments (kilobases to megabase)and, using efficient algorithms, provide high-quality assemblies in terms of contiguity and completeness of repetitive regions1,2,3,4. However, even though genome assemblies based on long reads exhibit high contig N50s (>1?Mb), these methods are still insufficient to decipher genome organization at the chromosome level. Here, we describe a strategy based on long reads (MinION or PromethION sequencers)and optical maps (Saphyr system)that can produce chromosome-level assemblies and demonstrate applicability by generating high-quality genome sequences for two new dicotyledon morphotypes, Brassica rapa Z1 (yellow sarson)and Brassica oleracea HDEM (broccoli), and one new monocotyledon, Musa schizocarpa (banana). All three assemblies show contig N50s of >5?Mb and contain scaffolds that represent entire chromosomes or chromosome arms.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHROMOSOME
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Belser, C.
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Personal name Istace, B.
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Personal name Denis, E.
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Personal name Dubarry, M.
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Personal name Baurens, F. C.
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Personal name Falentin, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deniot, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1b7qg0bJsemSrT_mojH96Vzm0LEbxyKVy/view?usp=drivesdk">https://drive.google.com/file/d/1b7qg0bJsemSrT_mojH96Vzm0LEbxyKVy/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18397 25.06.2025 25.06.2025 Documentos solicitados