A study of the distribution of apple stem pitting virus in tissues of pear tree using in situ hybridization and in situ RT-PCR. (Record no. 52567)

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control field MX-MdCICY
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control field 20250625162412.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18415
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245 10 - TITLE STATEMENT
Title A study of the distribution of apple stem pitting virus in tissues of pear tree using in situ hybridization and in situ RT-PCR.
490 0# - SERIES STATEMENT
Volume/sequential designation Agricultural Sciences in China, 8(11), p.1351-1359, 2009
520 3# - SUMMARY, ETC.
Summary, etc. To understand the distribution of Apple stem pitting virus (ASPV)in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were used as the materials, cDNA probe for ASPV was synthesized through RT-PCR reaction system using the unradioactive digoxigenin-labeled probe, and the specificity and sensitivity of probe were verified by blot hybridization method. Paraffin slice for in situ PCR and in situ hybridization was made and the location and distribution of ASPV RNA were detected in paraffin slices using in situ reverse transcription polymerase chain reaction, and the important factors which influenced the experiment results were optimized. ASPV mainly distributed in palisade tissue of mesophyll cells, external cortex of the tip, and the corresponding newborn vascular bundles. 20 min was the suitable digestive time for proteinase K. For the better amplication, RT reaction system should be above 0.2 U ?L?1and 0.4 mmol L?1 for RNasin and dNTPs respectively, 0.1-1.3 U ?L?1 SuperScript II, 0.6-0.8 ?mol L?1 primer concentration, and above 0.5 U 100 ?L?1 LA Taq DNA polymerase. The suitable annealing temperature in PCR reaction was 60°C with 35 cycles. The apical meristem of 0.25 mm was the region of virus-free.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element KORLA PEAR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element APPLE STEM PITTING VIRUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDNA PROBE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DIGOXIGENIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IN SITU PCR
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ying, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Na, L. I. U.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Niu, J. X.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1pWKuezGaBeFzE9-roSPk5Smizw5SAjRw/view?usp=drivesdk">https://drive.google.com/file/d/1pWKuezGaBeFzE9-roSPk5Smizw5SAjRw/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18415 25.06.2025 25.06.2025 Documentos solicitados