Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels. (Record no. 52611)

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fixed length control field 02170nam a2200265Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162412.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18460
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels.
490 0# - SERIES STATEMENT
Volume/sequential designation Plos one, 14.(8), p.e0220743, 2019
520 3# - SUMMARY, ETC.
Summary, etc. Decellularization of blood vessels is a promising approach to generate native biomaterials for replacement of diseased vessels. The decellularization process affects the mechanical properties of the vascular graft and thus can have a negative impact for in vivo functionality. The aim of this study was to determine how detergents under different fluid dynamics affects decellularization efficacy and mechanical properties of the vascular graft. We applied a protocol utilizing 1percent TritonX, 1 percent Tributyl phosphate (TnBP)and DNase on porcine vena cava. The detergents were applied to the vessels under different conditions; static, agitation and perfusion with 3 different perfusion rates (25, 100 and 400 mL/min). The decellularized grafts were analyzed with histological, immunohistochemical and mechanical tests. We found that decellularization efficacy was equal in all groups, however the luminal ultrastructure of the static group showed remnant cell debris and the 400 mL/min perfusion group showed local damage and tearing of the luminal surface. The mechanical stiffness and maximum tensile strength were not influenced by the detergent application method. In conclusion, our results indicate that agitation or low-velocity perfusion with detergents are preferable methods for blood vessel decellularization
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DECELLULARIZATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DISEASED VESSELS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Simsa, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vila, X. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Salzer, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Teuschl, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jenndahl, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bergh, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fogelstrand, P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1zTpdmUNR5DlKPEcgcvnDfHb4jJIj-Ss0/view?usp=drivesdk">https://drive.google.com/file/d/1zTpdmUNR5DlKPEcgcvnDfHb4jJIj-Ss0/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18460 25.06.2025 25.06.2025 Documentos solicitados