MARC details
| 000 -LEADER |
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| fixed length control field |
02862nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162413.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-18498 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Comparative transcriptome analysis reveals molecular regulators underlying pluripotent cell induction and callus formation in Anthurium andraeanum "Alabama". |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
In Vitro Cellular & Developmental Biology-Plant, p.1-13, 2021 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Shoot regeneration from pluripotent cell masses is an important process for genetic improvement of Anthurium andraeanum through Agrobacterium-mediated transformation. However, the mechanisms underlying pluripotent cell induction are not well understood. Here, three half-leaf explant samples collected after 0-, 2-, and 30-d culture on callus-inducing medium were used for RNA isolation and transcriptome profiling. In total, 225,752 non-redundant unigenes were assembled with a mean length of 1299 bp. Differentially expressed genes among the three samples were identified by comparative transcriptome analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG)analysis showed the greatest number of differentially expressed genes in the comparison between 0- and 2-d cultures were related to the "ribosome." Many differentially expressed genes in the comparison between 2- and 30-d cultures were involved in "plant hormone signal transduction," "starch and sucrose metabolism," and "phenylpropanoid biosynthesis." Further investigation was focused on genes related to transcription factors, epigenetic regulation, nitrous oxide metabolism, and homeostasis of plant hormones auxin, cytokinin, brassinolide, and strigolactone. Experiments were designed to confirm the effects of nitrous oxide and strigolactone on callus formation in Anthurium andraeanum. Callus formation was enhanced by exogenous application of sodium nitroprusside, which produces nitrous oxide, but was inhibited under rac-GR24 (a strigolactone analogue)treatment. Our comparative transcriptome analysis revealed that the signal molecule nitrous oxide and plant hormones auxin, cytokinin, and brassinolide act as positive regulators in promoting callus formation, while strigolactone may inhibit callus formation. Transcriptional and epigenetic regulations also play important roles in pluripotent cell induction in Anthurium andraeanum. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTHURIUM ANDRAEANUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLURIPOTENT CELL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CALLUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANSCRIPTOME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA-SEQ |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Li, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Li, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Li, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Guan, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Chen, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhu, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1yDzfZREvdTEnRsZT-K7slJH8mCCfutvF/view?usp=drivesdk">https://drive.google.com/file/d/1yDzfZREvdTEnRsZT-K7slJH8mCCfutvF/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
