MARC details
| 000 -LEADER |
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| fixed length control field |
02640nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162414.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-18543 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
An efficient mesophyll protoplast isolation, purification and PEG-mediated transient gene expression for subcellular localization in Chinese kale. |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Scientia Horticulturae, 241, p.187-193, 2018 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Transient gene expression serves as a valuable tool for gene functional study in plants. Here we reported protoplast isolation and purification from the plantlet young leaves of Chinese kale (Brassica oleracae var. alboglabra Bailey), and their transient gene expression with polyethylene glycol (PEG)-mediated transformation and subcellular localization of phytoene desaturase (BaPDS1). The procedures of isolation andtransformation ofmesophyll protoplasts derived from Chinese kale were optimized, and the influencing factors were analyzed. The results showed that the optimal protocol of protoplast isolation and purification was initialized by digestion in enzyme solution (2.0percent cellulase, 0.1percent pectolase, and 0.6?M mannitol)for 9?h. After filtered through 400 mesh and centrifuged at 179 ×g for purification, the total yield of protoplast reached as high as 6.04?×?107 protoplasts g?1 fresh weight (FW)and the viability of the protoplasts was up to 95percent. A maximum transformation efficiency of approximately 30percent measured by using green fluorescent protein (GFP)as a detecting gene was obtained when PEG4000 was at a final concentration of 40percent and transformation time was set to 15?min. In addition, the subcellular localization of BaPDS1 in Chinese kale was targeted to the chloroplast, confirming the efficiency and reliability of this transient transformation system. Taken together, an efficient protoplast isolation, purification and transformation system in Chinese kale was established in this study, laying a foundation for future research in molecular biology and gene function in Chinese kale and other Brassica vegetables. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHINESE KALE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTOPLAST |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANSIENT EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GREEN FLUORESCENT PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BAPDS1 |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Sun, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xiao, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jiang, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yuan, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xue, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tang, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1PSc-iQDKFi_Pd0yuAlBND3nVlnV86696/view?usp=drivesdk">https://drive.google.com/file/d/1PSc-iQDKFi_Pd0yuAlBND3nVlnV86696/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
