5-Azacytidine-removal of the dark repression in plastid development of Euglena gracilis Klebs. (Record no. 52701)

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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18551
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245 10 - TITLE STATEMENT
Title 5-Azacytidine-removal of the dark repression in plastid development of Euglena gracilis Klebs.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant physiology, 137(6), p.723-728, 1991
520 3# - SUMMARY, ETC.
Summary, etc. Asynchronous, etiolated cells of Euglena gracilis, a light-dependent organism for plastid differentiation, were grown in complete darkness for 72 h in the presence or absence of 5 ?g/mL 5-azaC, an inhibitor of DNA methylation. Plastid differentiation was followed by fluorescence and electron microscopy while the formation of Chl(ide)was ascertained by fluorimetric and HPLC analyses. It was found that in the presence of the compound, 20-40 percent of the cells was induced to form differentiated plastids, with kinetics greatly comparable to that of the wild-type dark-grown Euglena exposed to light. Based on the immediate or light-induced red-fluorescence of the organelles viewed under UV light it was established that in about 10 percent of the mentioned cells plastidogenesis reached the stage of Chl(ide)synthesis whereas in the other ones only the Chl(ide)precursors formed and a brief photoinduction was necessary for their assemblage. The removal of the dark repression of plastidogenesis operated by 5-azaC points to an involvement of DNA de(hypo)methylation in the expression of the genes responsible for plastid development as it occurs in other biological processes in both animals and plants. For the first time, Euglena gracilis was induced to form differentiated plastids in darkness
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EUGLENA GRACILIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 5-AZACYTIDINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA METHYLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASTIDOGENESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fasulo, M. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pancaldi, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bonora, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bruni, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dall'Olio, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1MUq_VbpvE5yqcqMzpFqhlQ9a7cDn9auJ/view?usp=drivesdk">https://drive.google.com/file/d/1MUq_VbpvE5yqcqMzpFqhlQ9a7cDn9auJ/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18551 25.06.2025 25.06.2025 Documentos solicitados