Heterologous expression systems for plant defensin expression: examples of success and pitfalls. (Record no. 52732)

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fixed length control field 01976nam a2200301Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162415.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18584
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Title Heterologous expression systems for plant defensin expression: examples of success and pitfalls.
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Volume/sequential designation Current Protein and Peptide Science, 18(4), p.391-399, 2017
520 3# - SUMMARY, ETC.
Summary, etc. Defensins are a superfamily of antimicrobial peptides, present in vertebrates, invertebrates, fungi and plants, suggesting that they appeared prior to the divergence in eukaryotes. The destitution of toxicity to mammalian cells of plant defensins has led to a new research ground, i.e., their potential medical use against human infectious diseases. Isolating defensins from natural sources, like plant tissues, can be time-consuming, labor intensive and usually present low yields. Strategies for large-scale production of purified active defensins have been employed using heterologous expression systems (HES)for defensin production, usually based in E. coli system. Like any other technology, HES present limitations and drawbacks demanding a careful experimental design prior the system selection. This review is proposed to discuss some of the major concerns when choosing to heterologously express plant defensins, with special attention on bacterial expression systems.
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Topical term or geographic name entry element E. COLI
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Topical term or geographic name entry element ANTIMICROBIAL PEPTIDES
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Topical term or geographic name entry element CARRIER PROTEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FUSION
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Topical term or geographic name entry element INCLUSION BODIES
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Topical term or geographic name entry element SECRETION SIGNAL
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Saldanha Gazzaneo,L. R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pandolfi, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Luiz Santos De Jesus,
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Crovella, S.
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Personal name Maria Benko-Iseppon,
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Personal name De Freitas, A. Carlos
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ASBhWHh9pHSEAQ4a7gEKGwrHVANXb1YF/view?usp=drivesdk">https://drive.google.com/file/d/1ASBhWHh9pHSEAQ4a7gEKGwrHVANXb1YF/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18584 25.06.2025 25.06.2025 Documentos solicitados